AUC KINANTHROPOLOGICA
AUC KINANTHROPOLOGICA

Acta Universitatis Carolinae Kinanthropologica (AUC Kinanthropologica) is an international peer reviewed journal for the publication of research outcomes in the humanities, the social sciences and the natural sciences, as applied to kinathropology. It is a multidisciplinary journal accepting only original unpublished articles in English in the various sub-disciplines and related fields of kinanthropology, such as Anthropology, Anthropomotorics, Sports Pedagogy, Sociology of Sport, Philosophy of Sport, History of Sport, Physiology of Sport And Exercise, Physical Education, Applied Physical Education, Physiotherapy, Human Biomechanics, Psychology of Sport, Sports Training and Coaching, Sport Management, etc. The journal also welcomes interdisciplinary articles. The journal also includes reports of relevant activities and reviews of relevant publications.

The journal is abstracted and indexed by CNKI, DOAJ, EBSCO, ERIH PLUS, SPOLIT, SPORTDiscus, and Ulrichsweb.

AUC KINANTHROPOLOGICA, Vol 59 No 1 (2023), 19–28

Reliability of the saliva self-sampling with and without supervision

Jana Jaklová Dytrtová, Radmila Dytrtová, Ishak Kovač, Michal Šteffl, Michal Jakl

DOI: https://doi.org/10.14712/23366052.2023.2
zveřejněno: 04. 07. 2023

Abstract

One of the least invasive sampling methods suitable for self-sampling is saliva spitting. The aim of this study is to evaluate the suitability of saliva self-sampling for unsupervised testing. Two self-sampling strategies were compared on the basis of visual evaluation of samples, measurement of cortisol levels in samples and questionnaire survey. The saliva samples obtained by supervised self-sampling were found to be fully suitable for further analysis. In contrast, not all saliva samples obtained from unsupervised self-collection can be used: 13% non-compliance with the minimum required sample volume, 8% with some food/drink residues and 26% taken at the wrong day time. About 42% of the unsupervised probands made at least one significant error in the saliva self-collection procedure. These results indicate that the accuracy of the results based on the analysis of samples received from saliva self-sampling is limited. For clinical investigation, the presence of an inner standard (referring to the reliability of the sampling procedure) is required.

klíčová slova: saliva sampling; cortisol responsibility; biosensors; hormones; immunoassays

reference (15)

1. Bellagambi, F. G., Lomonaco, T., Salvo, P., Vivaldi, F., Hangouet, M., Ghimenti, S., Biagini, D., Di Francesco, F., Fuoco, R., & Errachid, A. (2020). Saliva sampling: Methods and devices. An overview [Review]. Trac-Trends in Analytical Chemistry, 124, Article 115781. CrossRef

2. Bhattarai, K. R., Kim, H. R., & Chae, H. J. (2018). Compliance with saliva collection protocol in healthy volunteers: Strategies for managing risk and errors [Review]. International Journal of Medical Sciences, 15(8), 823-831. CrossRef

3. Broderick, J. E., Arnold, D., Kudielka, B. M., & Kirschbaum, C. (2004). Salivary cortisol sampling compliance: comparison of patients and healthy volunteers [Article]. Psychoneuroendocrinology, 29(5), 636-650. CrossRef

4. Bulthuis, M. S., Jager, D. H. J., & Brand, H. S. (2018). Relationship among perceived stress, xerostomia, and salivary flow rate in patients visiting a saliva clinic [Article]. Clinical Oral Investigations, 22(9), 3121-3127. CrossRef

5. Fries, E., Dettenborn, L., & Kirschbaum, C. (2009). The cortisol awakening response (CAR): Facts and future directions [Review]. International Journal of Psychophysiology, 72(1), 67-73. CrossRef

6. Gröschl, M. (2017). Saliva: a reliable sample matrix in bioanalytics [Review]. Bioanalysis, 9(8), 655-668. CrossRef

7. Guthrie, J. T., Wigfield, A., Barbosa, P., Perencevich, K. C., Taboada, A., Davis, M. H., Scafiddi, N. T., & Tonks, S. (2004). Increasing reading comprehension and engagement through concept-oriented reading instruction [Article]. Journal of Educational Psychology, 96(3), 403-423. CrossRef

8. Kudielka, B. M., Broderick, J. E., & Kirschbaum, C. (2003). Compliance with saliva sampling protocols: Electronic monitoring reveals invalid cortisol daytime profiles in noncompliant subjects [Article]. Psychosomatic Medicine, 65(2), 313-319. CrossRef

9. Ljubijankic, N., Popovic-Javoric, R., Sceta, S., Sapcanin, A., Tahirovic, I., & Sofic, E. (2008). Daily fluctuation of cortisol in the saliva and serum of healthy persons [Article]. Bosnian Journal of Basic Medical Sciences, 8(2), 110-115. CrossRef

10. Miller, R., Stalder, T., Jarczok, M., Almeida, D. M., Badrick, E., Bartels, M., Boomsma, D. I., Coe, C. L., Dekker, M. C. J., Donzella, B., Fischer, J. E., Gunnar, M. R., Kumari, M., Lederbogen, F., Power, C., Ryff, C. D., Subramanian, S. V., Tiemeier, H., Watamura, S. E., & Kirschbaum, C. (2016). The CIRCORT database: Reference ranges and seasonal changes in diurnal salivary cortisol derived from a meta-dataset comprised of 15 field studies [Article]. Psychoneuroendocrinology, 73, 16-23. CrossRef

11. Powell, D. J. H., Liossi, C., Moss-Morris, R., & Schlotz, W. (2013). Unstimulated cortisol secretory activity in everyday life and its relationship with fatigue and chronic fatigue syndrome: A systematic review and subset meta-analysis [Review]. Psychoneuroendocrinology, 38(11), 2405-2422. CrossRef

12. Raff, H. (2009). Utility of salivary cortisol measurements in Cushingʼs syndrome and adrenal insufficiency [Review]. Journal of Clinical Endocrinology & Metabolism, 94(10), 3647-3655. CrossRef

13. Roblegg, E., Coughran, A., & Sirjani, D. (2019). Saliva: An all-rounder of our body [Review]. European Journal of Pharmaceutics and Biopharmaceutics, 142, 133-141. CrossRef

14. Taylor, J. L., & Corbett, B. A. (2014). A review of rhythm and responsiveness of cortisol in individuals with autism spectrum disorders [Review]. Psychoneuroendocrinology, 49, 207-228. CrossRef

15. Thieme, D. (2012). Potential and limitations of alternative specimens in doping control [Review]. Bioanalysis, 4(13), 1613-1622. CrossRef

Creative Commons License
Reliability of the saliva self-sampling with and without supervision is licensed under a Creative Commons Attribution 4.0 International License.

157 x 230 mm
vychází: 2 x ročně
cena tištěného čísla: 190 Kč
ISSN: 1212-1428
E-ISSN: 2336-6052

Ke stažení